Pediococcus acidilactici TAK 589 Coccobest
Species Pediococcus acidilactici is a widespread member of the genus Pediococcus. This species is found in plant material, milk, and meat products, and in the gastrointestinal tract of mammals, and fish.
In a real-life situation (newborn calves) Pediococcus acidilactici TAK 589 Coccobest has been shown to have a positive effect on the mammalian microbiota (reduction of the Enterobacteriaceae and, specifically, E. coli and coliform microorganisms of this family) and significant reduction of cases of bacterial infections and prevention of fatal outcome associated with more severe infections. Thus, Pediococcus acidilactici TAK 589 Coccobest provides local gastrointestinal protection by balancing a mammalian gastrointestinal microbiota, suppressing bacterial infections causing pathogens, and thereby helping to prevent and / or reduce diarrhea and its serious complications (including death of young animals).
Zootechnological additives | TAK 59 | BioCC 203 | CoccobEst® | BioCC 228 | E-98
Prolonged and excessive formation of reactive particles (e.g., free radicals) in a mammal causes oxidative stress, promotes inflammatory processes and is involved in the development and progression of many diseases. In addition to other factors, microbiota and intestinal mucosal interactions can cause oxidative stress, which impairs the protective barrier function of the gastrointestinal mucosa and in turn leads to systemic physiological changes (i.e., to changes that are not limited to the gastrointestinal tract).
Thus, the neutralisation of reactive particles is of utmost importance. Strain Pediococcus acidilactici TAK 589 Coccobest possesses strong antioxidative activity, which protects this microbe against excessive oxidative stress. Extracellular antioxidative biomolecules produced by P. acidilactici TAK 589 Coccobest and absorbed from the gastrointestinal tract into the bloodstream help to neutralize reactive particles and reduce oxidative stress in mammalian body cells.
Thus, in addition to the local protection of the mammalian gastrointestinal tract, P. acidilactici TAK 589 Coccobest is also able to provide additional systemic (whole-body-based) protection against and / or reduction of excessive oxidative stress and consequent inflammatory processes.
Additionally, P. acidilactici TAK 589 Coccobest is suitable for use as a technological starter culture for fermentation of vegetables. The strong antimicrobial activity of P. acidilactici TAK 589 Coccobest also helps the microbe to act as a protective culture for suppressing food-borne pathogens in food product.
P. acidilactici TAK 589 Coccobest has good temperature tolerance at sublethal temperatures (60 ° C and 70 ° C). The good temperature tolerance of P. acidilactici TAK 589 Coccobest and the preservation of biological activity at higher temperatures gives the strain an advantage of using it both as a zootechnical feed additive (incl. with whole milk replacer for calves) and as a technological starter culture in the food.
Pediococcus acidilactici TAK 589 Coccobest is a multipotent microorganism strain, possessing several functional properties with synergistic effects.
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Equally strong antimicrobial activity against pathogens in both microaerobic and anaerobic environments
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Preservation of bioactivity at higher temperatures, including sublethal temperatures, and good compatibility with food matrix in combination with antimicrobial activity allows Pediococcus acidilactici TAK 589 Coccobest to be used as a zootechnical feed additive (veterinary probiotic) to prevent bacterial infections in the gastrointestinal tract of farm animals and pets and to prevent and reduce diarrhea and to prevent and / or reduce oxidative stress both and as a technological starter culture for fermentation of vegetables and as a protective culture for suppressing food-borne pathogens in food product.
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Antioxidant activity combined with antimicrobial activity provides local protection to the mammalian body in the gastrointestinal tract (suppression of gram-negative and gram-positive pathogens, thereby balancing the microbiota and strengthening the gastrointestinal mucosal protective barrier function) and additional whole-body-based systemic protection against and / or reduction of excessive oxidative stress and consequent inflammatory processes.
Functional properties of Pediococcus acidilactici TAK 589 Coccobest
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Strain P. acidilactici TAK 589 Coccobest has no resistance against ampicillin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, chloramphenicol.
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Strain P. acidilactici TAK 589 Coccobest has antioxidant activity on both intact microbial cells and cell lysate.
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The strain P. acidilactici TAK 589 Coccobest has good temperature tolerance: the viable counts do not change at 60 °C for 10-20 minutes and decreases by one logarithm at 70 °C.
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P. acidilactici TAK 589 Coccobest inhibits the growth of the enteropathogens (e.g., L. monocytogenes, E. coli, S. enteritidis, Salmonella enterica serovar Typhimurium, S. sonnei, S. aureus, E. faecalis and Cronobacter sakazakii).
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Animal trial. Administration of P. acidilactici TAK 589 Coccobest to newborn cow calves reduced the number of E. coli in the faecal samples of the experimental group by one logarithm by day 30. The number of Coli-like microbes and enterobacteria decreased in the faecal samples of the experimental group by 1.1 and 1.3 logarithms, respectively, by day 30 of life. The E. coli abundance in the faecal samples of the control group increased by 0.5 logarithm by the 30th day of life. The number of coliforms and enterobacteria in the faecal samples of the control group increased by 0.4 logarithm by day 30. Diarrhea occurred in seven calfs out of 16 (44%) in the control group and only three out of 16 (19%) in the experimental group. Administration of P.acidilactici TAK 589 Coccobest reduced the number of E. coli in the faecal samples of the experimental group by 1.6 logarithms for the fifth day of life and by 2.5 logarithms for the eighth day. The number of Coli-like microbes in the faecal samples of the experimental group decreased by 0.3 logarithm by day 5 and by 0.8 logarithm by day eight. Between days 15.-56., diarrhea occurred in only one of 16 calves in the experimental group (6.3%) and in seven of the 16 calves in the control group (43.8%). Two calves died in the control group.